Fig. 7: Biomedical application of hybrid exosomes for drug delivery across the BBB.
From: Fusogenic lipid nanoparticles for rapid delivery of large therapeutic molecules to exosomes
a Confocal microscopy images showing the permeability of doxorubicin, IgG, and mRNA when delivered via cubosomes (top) and hybrid exosomes (bottom) across the BBB model. Scale bar: 200 μm. b Quantitative analysis of the area of fluorescence intensity for doxorubicin (Dox), IgG, and mRNA delivered using cubosomes and hybrid exosomes. Hybrid exosomes significantly enhance the transport of IgG and mRNA across the BBB compared to cubosomes. Data are presented as mean values with SD. Statistical significance: ****p < 0.0001 (mRNA: n = 6, Dox and IgG: n > 8; n represents the number of independent experiments). Statistical significance was determined using an ordinary one-way ANOVA followed by Tukey’s multiple comparisons test,. c Confirmation of intact mRNA transport across the BBB. Texas Red-labeled hybrid exosomes (red) successfully encapsulate and deliver mRNA (yellow) across the endothelial barrier. The merged image shows the colocalization of hybrid exosomes and mRNA beyond the BBB boundary. Scale bar: 20 μm.
