Fig. 5: Regulation of neonatal SCs differentiation.
From: Gonadotrophs have a dual origin, with most derived from early postnatal pituitary stem cells
A Differentiation was induced in pituispheres from P5 (top) or 7-week-(bottom) old male pituitaries. The presence of differentiated cells within spheres was evaluated by immunofluorescence using LH or collectively GH, PRL and TSH antibodies for POU1F1 lineage cell detection. SOX2 staining confirms the presence of pituisphere forming-cells. B The proportion of spheres containing LH or POU1F1 lineages cells were compared between ages in both sexes. Multiple two-tailed unpaired-tests with Holm-Å Ãdák post hoc test, cultures from N = 3 animals per sex and per age (for females, adj.p = 0.0002 at P5 and 0.0097 in adults; for males adj.p = 0.07 at P5 and 0.004 in adults). C Timeline of GnRH antagonist (cetrorelix) administration and lineage tracing induction (Dox) in Sox2rtTa;eYFP pups. D Immunofluorescence for eYFP and LH in pituitaries of P33 cetrorelix treated male and control mice showing no apparent differences in SC-derived gonadotroph emergence. E,F Quantification in both sexes at P33 shows no effect of cetrorelix on either the proportion of LH+ve/DAPI+ve cells (E) or the proportion of eYFP; LH double+ve/LH+ve cells (F). G Timeline of gonadectomy and lineage tracing induction (Dox) in Sox2rtTa;eYFP pups. H) Immunofluorescence for eYFP and LH in pituitaries of P21 gonadectomised male and sham-operated control showing no apparent differences in SC-derived gonadotroph emergence. I–J Quantification in both sexes at P21 shows no effect of gonadectomies on either the proportion of LH+ve/DAPI+ve cells (I) or eYFP; LH double+ve/LH+ve cells (J). K Timeline of androgen antagonist (flutamide) treatment and lineage tracing induction (Dox) in Sox2rtTa;eYFP pups. Created in BioRender. Rizzoti, K. (2025) https://BioRender.com/ uge0o92. L,M Quantification in males at P21 shows no effect of flutamide on either the proportion of LH+ve/DAPI+ve cells (L) or the proportion of eYFP; LH double+ve/LH+ve cells (M). Scale bar = 80 μm for all images. On graphs, each dot represents one animal with median values indicated. Source data are provided as a Source Data file.
