Fig. 5: Co-transcription and Transcriptional Interference (TI) at the Xist/Tsix Locus. | Nature Communications

Fig. 5: Co-transcription and Transcriptional Interference (TI) at the Xist/Tsix Locus.

From: Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist–Tsix locus

Fig. 5

a Diagram of the Xist/Tsix locus showing Xist exons (green), Tsix exons (magenta), introns (lines), and probe positions for Xist introns (green) and Tsix 3’ introns (magenta). Polymerase positions suggest potential co-transcription of Xist and Tsix. b Conceptual diagram showing expected outcomes with or without TI. If TI occurs, Xist (green) and Tsix 3’ (magenta) signals won’t colocalize (left). Without TI, colocalization occurs (right). c Example image showing DAPI (blue), Xist (green), Tsix 3’ (magenta), and colocalization (white). d–f Similar diagrams and images as (a-c), but for Tsix 5’. With TI, more Xist and Tsix 5’ colocalization occurs than with Tsix 3’ (left); without TI, colocalization remains consistent (right). g, h Joint probability distributions of nascent Tsix (x-axis) and Xist (y-axis) transcripts. Measured data is on the left, randomized data on the right. Rho and p values indicate Spearman correlations (real data) and two-dimensional two sided KS test results (randomized data). Analysis includes 625 cells for Tsix 5’ and 651 cells for Tsix 3’.

Back to article page