Fig. 6: Co-transcription and Transcriptional Interference (TI) at the Xist/Tsix Locus.

a–f Quantification of colocalization at transcription sites, varying by nascent transcription levels (low, medium, high). Colocalization is plotted by search radius (x-axis, cubic scale) and average events per transcription site (y-axis). The average colocalization is calculated for each replicate. The solid line represents the mean, while the shaded area indicates the standard deviation, based on data from three independent biological replicates (N = 3). Dashed lines is the background fit. Dotted line is the colocalization by chance. a, c, e Quantification of colocalization of Xist with Tsix 3’ depending on the nascent transcription of Xist. Low-level nascent Xist (a), shows colocalization with Tsix 3’ (18 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 3’ (54 tsites) (c), and a lower fraction of high-level Xist colocalizes with Tsix 3’ (11 tsites) (e). A total of n = 651 cells are quantified in this analysis, with Replica 1, N = 118 cells, Replica 2, N = 276 cells, Replica 3, N = 256 cells. b, d, f Quantification of colocalization of Xist with Tsix 5’ depending on the nascent transcription of Xist. Low-level nascent Xist (b), shows colocalization with Tsix 5’ (30 tsites). A greater fraction of medium-level Xist colocalizes with Tsix 5’ (68 tsites) (d), and similarly high fraction of high-level Xist colocalizes with Tsix 5’ (33 tsites) (f). A total of n = 625 cells are quantified in this analysis, with Replica 1, N = 145 cells, Replica 2, N = 259 cells, Replica 3, N = 221 cells).