Fig. 8: Assessment of KAT5 activity, AHA incorporation, and homoharringtonine sensitivity.
From: KAT5 regulates neurodevelopmental states associated with G0-like populations in glioblastoma
a Flow-based assessment of H4-Ac and AHA incorporation in NSCs, transformed NSCs and GSC-0827 cells. b Homoharringtonine (HHT) sensitivity of NSCs, transformed NSCs, and GSCs. The approximate IC50 (100 nM) for NSC-CB660 cells was used for this assay. Cells were treated for 4 days 100 nM HHT (the ~IC50 of NSC-CB660 cells) after which viability was measured (n ≥ 3). Means and ± SEMs are shown, unpaired, 2 tailed t-tests were used for significance. Supplementary Fig. 16 shows MYC and MYCN expression as well as H4-Ac and AHA incorporation for GSC-464T and GSC-1406. c Model of KAT5 function in HGG and LGG tumors arising from this work. LGGs have lower levels of KAT5 activity and cell cycle genes but appear to maintain similar levels of MYC, MYCN, and KAT5 transcripts as HGGs (Supplementary Fig. 16). If true, one possibility is that the presence of excessive cellular 2-hydroxyglutarate, produced by oncogenic IDH1/2 mutant enzymes, keeps KAT5 activity. Created in BioRender. Paddison, P. (2025) https://BioRender.com/6xzam1p. Source data and exact p values are provided with this paper as a Source Data file.
