Fig. 1: L704F mutation in ABCG36 uncouples IBA from camalexin export.
IBA (a) CLX (b) and IAA (c) export from Arabidopsis protoplasts prepared from indicated ABCG36 loss-of-function alleles. Significant differences (p < 0.05) of means ± SE (n = 8 independent protoplast preparations) were determined using Ordinary One-way ANOVA (Tukey’s multiple comparison test) and are indicated by different lowercase letters. IBA (d) CLX (e) and IAA (f) export from N. benthamiana protoplasts after transfection with indicated mutant versions ABCG36. Significant differences (p < 0.05) of means ± SE (n = 14 independent protoplast preparations) were determined using Ordinary One-way ANOVA (Tukey’s multiple comparison test) and are indicated by different lowercase letters. Confocal imaging (g) and quantification (h) of GFP-tagged versions of mutated ABCG36 after transfection of N. benthamiana leaves. Short treatment of FM4-64 was used as PM markers; bar, 50 μm. Significant differences (p < 0.05) of means ± SE (n = 25 epidermal cells) were determined using Ordinary One-way ANOVA (Tukey’s multiple comparison test) and are indicated by different lowercase letters. i ATPase activity of microsomal fractions prepared from tobacco leaves transfected with vector control or indicated mutant versions of ABCG36 measured at pH 9.0 in the presence of 50 μM indole, IAA, IBA, CLX, or ortho-vanadate. Significant differences (p < 0.05) of means ± SE (n = 3 independent transfections and microsomal preparations) were determined using Ordinary Two-way ANOVA (Tukey’s multiple comparison test) and are indicated by different lowercase letters. Data are presented as box-and-whisker plots, where median and 25th and 75th percentiles are represented by the box itself and the middle line, respectively; means are indicated by a “+”. Source data is provided as a Source Data file.
