Fig. 3: TSP4a and TSP4b comprise a positive feedback loop activated in response to heat stress and auxin cues.

a TSP4a and TSP4b expression in –2 DPA ovaries isolated from plants carrying alleles with different combinations of TSP4a and TSP4b haplotypes grown under high temperature. b TSP4a and TSP4b expression in –2 DPA ovaries isolated from Moneymaker, tsp4bcr single mutants, tsp4acr single mutants and tsp4a/4bcr double mutants grown under high temperature. c Time course of TSP4a and TSP4b expression at 25 °C and 35 °C. Seedlings were grown to the 4-leaf stage at 25 °C under 12-h light/12-h darkness and then transferred into 25 °C temperature and constant illumination for 5 d. Afterward, seedlings were transferred to 35 °C and constant illumination. Leaf samples were collected at the indicated time points for analysis. d TSP4a and TSP4b expression in Moneymaker, tsp4acr and tsp4bcr single mutants after exposure to 25 °C and 35 °C for 2 h. e Time course of TSP4a and TSP4b expression after mock and 10 μM IAA treatment. Twelve-day-old seedlings were incubated on 1/2 MS medium under 25 °C and 12-h light/12-h dark in a growth chamber. Seedlings were transferred to liquid 1/2 MS medium with or without 10 μM IAA. Samples were collected and analyzed at the indicated time points. f TSP4a and TSP4b expression in Moneymaker and tsp4bcr single mutants or tsp4acr single mutants treated with 10 μM IAA for 2 h. Data in (a–f) are the mean ± S.D. of n = 3 biologically independent replicates and relative expression was normalized to UBIQ3. Data in (a–c, e) were determined by one-way ANOVA with multiple comparisons and Tukey post-hoc test (α = 0.05). **P < 0.01. Data in (d, f) were determined by two-way ANOVA with multiple comparisons and Tukey post-hoc test (α = 0.05). **P < 0.01. Source data are provided as a Source Data file.