Fig. 7: HypnoS monitors iAdo dynamics in freely moving mice during seizures.
a Schematic diagram depicting the strategy for in vivo fiber photometry recording during the seizures. b Example traces of simultaneous EEG (scale, 0.5 mV), EMG (scale, 0.5 mV), HypnoS (top, green) and HypnoS-mut (bottom, gray) signals (scale, 50% ΔR/R0) measured during the indicated seizure stages. c Peak responses of HypnoS in twin seizures. n = 6 mice for each treatment. Paired two-tailed Student’s t-test: P = 0.037 between the first and the second seizure. d Peak responses of HypnoS and HypnoS-mut. n = 6 mice for each treatment. Paired two-tailed Student’s t-test: P = 0.00020 between saline and KA for HypnoS. e HypnoS signals aligned to seizure termination for all mice. (Left panel) Heat map plot of 6 individual trials; (right panel) averaged signal; red line, seizure termination. n = 6 trials from 6 mice. f Summary of rise and decay time constants measured for the fluorescence change of HypnoS to seizures. n = 13 seizure events in 6 mice. Two-tailed Student’s t-test: P = 5.3 × 10−8 between τon and τoff. Data are shown as mean ± s.e.m. in e, f with the error bars or shaded regions indicating the s.e.m. * P < 0.05; ***P < 0.001. Source data are provided as a Source Data file.
