Fig. 2: Preparation, catalysis and motion properties characterization of RDN@PL.
From: Reactive oxygen species responsive nanomotors for gene edited metabolic disruption and immunotherapy
a TEM images of morphological changes over time after the formation of RDN vesicles (Scale bar: 100 nm). The experiment was repeated independently three times with similar results, and a representative result is shown. b UV–Vis absorption spectra of different samples. c The DNA gel electrophoresis image of the free plasmids after electroporation, the concentration ratio is RDN to plasmid (N/P), and (d) The encapsulation efficiency and loading ratio of plasmids in RDN@PL (n  =  3 independent experiments). e The TEM image of RDN@PL (Scale bar: 200 nm). The experiment was repeated independently three times with similar results, and a representative result is shown. f The hydration diameter and (g) zeta potential of different particles (n  =  3 independent experiments). h Steady-state kinetic assay of different particles for the catalyzation of H2O2. The motion trajectories of (i) Non-RDN@PL and (j) RDN@PL recorded by bright field microscope (Scale bar: 10 μm). k Finite element simulation for the catalytic reaction of RDN@PL, which considers the release site, production rate, and diffusion rate of O2. l The Mean square displacement (MSD) of Non-RDN@PL and RDN@PL versus time interval (Δt) in H2O2 solution with different concentrations. m The diffusion coefficients statistical analysis of particles in H2O2 solution (n  =  15 independent experiments). n The clearance efficiency of Non-RDN@PL and RDN@PL for ·OH within 30 min (n  =  3 independent experiments). Data are presented in the form of mean values ± standard deviation (SD). Source data are provided as a Source Data file.
