Fig. 3: Chemotaxis and intracellular activity studies of RDN@PL on tumor cells.
From: Reactive oxygen species responsive nanomotors for gene edited metabolic disruption and immunotherapy
a Schematic illustration of the Transwell model to assess the Z-axis chemotaxis of RDN@PL towards B16F10 cells. b The fluorescence intensity representing particles content in the upper and lower chambers after treatment for different time (t = 0, 1, 3, 6, 12 h) in Transwell model. c Schematic illustration of the Y-shaped microchannel model to assess the X–Y axis chemotaxis of RDN@PL on B16F10 cells and chemotaxis induction on Raw264.7 cells. d The directional distribution of particles’ movement trajectories at the channel bifurcation (Left, n  =  50 independent samples) and the statistical analysis of fluorescence intensity in different chambers of Y-shaped microchannel (Right, n  =  3 independent experiments). e The 2D (Left) and 3D (Right) CLSM imaging of different particles uptake by B16F10 cells after incubation for 6 h (Scale bar: 20 μm). CLSM images (Left, scale bar: 10 μm) and fluorescence co-localization analysis of (f) lysosomes and (g) mitochondria of B16F10 cells with Cy5.5-labeled particles after incubation for 3 h (Yellow rectangles in the histograms mark the fluorescence overlap areas, purple rectangles mark non-overlap areas). The CLSM images of RDN@PL in (h) B16F10 cells and (i) HUVECs (Scale bar: 10 μm), and the corresponding statistical analysis of Pearson’s R value for co-localization (n  =  5 independent experiments), RDN and plasmid were labeled by Cy5.5 and YOYO-1, respectively (Scale bar: 10 μm). Data are presented in the form of mean values ± standard deviation (SD). Source data are provided as a Source Data file.
