Fig. 5: Assessment of the RDN@PL’s regulation on lactate metabolism and intra/extra-cellular ROS of B16F10 cells.
From: Reactive oxygen species responsive nanomotors for gene edited metabolic disruption and immunotherapy
a Western blotting analysis of LDHA after treatment. b Fluorescence images of intracellular CO levels after 24 h treatment (Scale bar: 200 μm). Statistical analysis of (c) ATP and (d) lactate content of B16F10 cells after 24 h treatment (n = 3 independent experiments), and (e) corresponding cell viability (n = 3 independent experiments). f Flow-cytometric analysis of the dead and alive staining of B16F10 cells. g Illustration of the mechanism that RDN@PL regulates tumor cell metabolism and intra/extra-cellular ROS. h The CLSM images of tumor cells mitochondria stained with JC-1 after treatments (Scale bar: 50 μm), and corresponding (i) fluorescence ratio analysis (n = 3 independent experiments). j The inter/external H2O2 level of HUVECs after treatments (n = 3 independent experiments). k–o The H2O2 level of inter-external of B16F10 cells after treatments with different time (n = 3 independent experiments), and the corresponding (p) statistical analysis of H2O2 level at t = 8 h (n = 3 independent experiments). Data are presented in the form of mean values ± SD. Significance was assessed via one-way ANOVA with Tukey’s post hoc test. Source data are provided as a Source Data file.
