Fig. 3: Exploring the effects of precursor overaccumulation and pathway activation on C20:5 synthesis in Y. lipolytica. | Nature Communications

Fig. 3: Exploring the effects of precursor overaccumulation and pathway activation on C20:5 synthesis in Y. lipolytica.

From: Regulating triacylglycerol cycling for high-efficiency production of polyunsaturated fatty acids and derivatives

Fig. 3

a Schematic of the metabolic pathways related to FA biosynthesis in Y. lipolytica. G6P 6-phospho gluconate, FBP fructose bisphosphate, G3P glycerol-3-phosphate, OAA oxaloacetate, α-KG α-ketoglutarate, FAS fatty acid synthesis, GUT2 glycerol-3-phosphate dehydrogenase, GPD1 glycerol-1-phosphate dehydrogenase, GPAT glycerol-3-phosphate acyl-transferase, LPAAT lysophosphatidate acyl-transferase, DGAT diacylglycerol acyl-transferase, TGL3/TGL4 triacylglycerol lipase, PEX10 peroxisomal biogenesis factor 10, POX peroxisomal acyl-CoA oxidase. b Analysis of the effects of C16:0 overaccumulation on the C20:5 biosynthesis. Glucose was removed at 168 h, the red line represents the fatty acid content in the absence of glucose, and the blue line represents the fatty acid content in the presence of glucose. The red arrow represents the time point of glucose deficiency. c Analysis of the transcription levels of seven desaturase/elongase genes related to C20:5 biosynthesis at 144, 192, and 240 h. b, c Data are presented as the means ± s.d. from three (n = 3) biologically independent replicates. Statistical analysis was performed using two-tailed Student’s t-test (*P < 0.05).

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