Fig. 5: EE transport towards the growing hyphal tip in T. reesei.

a EEs, labelled with TrmsGFP-Rab5, in a 1^st cell. Scale bar = 20 µm; plasma membrane is labelled by TrmCherry-Sso1 (red). b Motility of EEs (TrmsGFP-Rab5) in a 4^th cell; diffusional motility indicated by filled arrowhead, directed motility indicated by open arrowhead. Horizontal scale bar = 2 µm; vertical scale bar = 3 s. See also Supplementary Movie 9. c Quantitative analysis of anterograde motility of EEs in the 1^st cell (dark blue) and following subapical cells; measurements in subapical cells were done 10 µm behind the septum; numbers in the 1^st cells were determined 25 µm behind the apex. Sample size n = 41 for 1^st cell, n = 22 for 2^nd cell, n = 36 for 3^rd cell n = 35 for 4^th and 5^th cell and n = 37 for 6^th cell from 2 independent experiments. d Contrast-inverted kymographs showing the effect of microtubule inhibitor benomyl or the F-actin inhibitor latrunculin A (LatA) on EE motility. Cells were treated for 30 min with 10 µM inhibitor or corresponding amounts of the solvent DMSO (control). Horizontal scale bar = 1.5 µm; vertical scale bar = 3 s. See also Supplementary Movie 10. e Kymograph showing motility of EEs (TrmsGFP-Rab5; green), across the 1^st septum, labelled with the plasma membrane marker TrmCherry-Sso1 (red). Horizontal scale bar = 2 µm; vertical scale bar = 3 s. See also Supplementary Movie 11. f Quantitative analysis of directed anterograde motility of EEs across septa. Sample size n = 22 for 1^st septum, n = 35 for 2^nd and 3^rd septum, n = 34 for 4^th septum and n = 37 for 5^th septum from 2 independent experiments. Results shown in (a, b, d, e) were confirmed in 2 independent experiments. Bars in (c, f) represent mean ± SEM; sample sizes are indicated. Data in (a–f) were obtained from growing hyphae. All data are provided in the Source Data file.