Fig. 9: The role of EE motility in hyphal growth and graphical summary.
a Maximum likelihood tree of putative Hook adaptor proteins, reported to bind motor proteins to EEs67,68. NCBI accession numbers given in parenthesis; tree was generated using MEGA5.273. b Contrast-inverted kymographs showing EE motility in wildtype strain QM6a and in a hok1 deletion mutant. Horizontal scale bar = 2 µm; vertical scale bar = 3 s. See also Supplementary Fig. 10. c Hyphal growth rate in strain QM6a (Wildtype) and a Trhok1 deletion mutant (Δhok1). Sample size n = 31 hyphae from 2 independent experiments. d Morphology of wildtype strain QM6a and a Trhok1 null mutant. Deletion of the endosomal motor adaptor gene hok1 results in increased branching, which is probably a consequence of reduced hyphal growth rate. Open arrowheads indicate leading hypha, closed arrowhead indicates subapical branching. Cells were grown on agar plates. Scale bar = 100 µm. e Graph summarising the contribution of the apical and following 5 subapical cells in hyphal growth of T. reesei hyphae. Note that motility of SVs (blue bars) and EEs (red bars) through septa is given; drift of EEs due to CS (green bars) was measured within cells; the length of each bar represents the relative amount found in tip cells (20–30 µm behind the apex). The length of each cell is drawn to scale. f Model of the contribution of subapical secretion and endocytic recycling in tip extension. Marker genes, used to label compartments, vesicles, microtubules and cargo, are included. Results shown in (b, c) were confirmed in 2 independent experiments. Bars in (c) represent mean ± SEM; sample sizes are indicated. Statistical comparison with in (c) used Student’s t testing testing; ****= two-tailed P-values < 0.0001. All data are provided in the Source Data file.
