Fig. 2: Unsupervised phenotyping of microvascular leukocytes in the healthy mouse kidney.
From: Microvascular immunity is organ-specific and remodeled after kidney injury in mice
a, b Single cell sequencing of leukocytes sorted from blood, the renal microvasculature (MV) and the renal tissue. UMAP visualization and annotation of 27 leukocyte subtypes. n = 4 independent experiments. c Abundance of cell cluster specific leukocytes in the three compartments. Blue is the lowest number and red the highest number for each column. The top bar graphs represent the total cell count of each cluster as mean ±+/− SD of n = 4 independent experiments. d Ranked fold change of microvascular versus peripheral blood (top), and microvascular versus tissue-resident (bottom) leukocytes for each cell cluster. e Selected cell clusters are shown in the three compartments as fraction of all detected leukocytes. Unpaired two sided t test between MV and blood, and MV and tissue. All clusters are shown in supplementary Fig. 4. Mean and SD are shown. Each dot represents one independent experiment. Source data are provided as a Source Data file.
