Fig. 2: Netrin-1 deletion in postnatal cerebellar granule neuron progenitors does not impair SHH MB formation.

a Representative image of in situ hybridization of Ntn1 mRNA and immunostaining of netrin-1 and CaBP, in P7 mouse cerebellum. The nuclei were stained with DAPI (scale bar: 50 µm). b Genotyping obtained by PCR on Atoh1^CreERT2+; Ptch1^flox/flox mice depending on Ntn1 expression: Ntn1^wt/wt, Ntn1^flox/wt and Ntn1^flox/flox. c Kaplan-Meier survival curve of tamoxifen treated-Atoh1^CreERT2+; Ptch1^flox/flox mice depending on Ntn1 expression: Ntn1^wt/wt (n = 8) compared to Ntn1^flox/wt (n = 16) and Ntn1^flox/flox (n = 8) (ns: not significant). d Representative images of SHH MB developed in tamoxifen treated-Atoh1^CreERT2+; Ptch1^flox/flox mice depending on Ntn1 expression: Ntn1^wt/wt and Ntn1^flox/flox. Atoh1^CreERT2− mice were used as a control. Cerebellum slices were stained with Hematoxylin and Eosin (H&E), and Ki-67 immunostaining was performed (scale bar: 500 µm). e Gli1, Gli2 and Atoh1 mRNA expression analyzed by quantitative PCR in cerebellum from tamoxifen treated-Atoh1^CreERT2+; Ptch1^flox/flox mice depending on Ntn1 expression: Ntn1^wt/wt (n = 6), Ntn1^flox/wt (n = 8) and Ntn1^flox/flox (n = 8). Atoh1^CreERT2− (n = 5) mice were used as a control. The results are expressed as mean ± SEM. (two-tailed Mann-Whitney test, compared to Atoh1^CreERT2− group: Ntn1^wt/wt **p = 0.0087 (Atoh1), *p = 0.0426 (Gli1) = 0.0303 (Gli2); Ntn1^flox/wt **p = 0.0047 (Gli1) = 0.0027 (Gli2) = 0.001 (Atoh1); Ntn1^flox/flox **p = 0.0047 (Gli1) = 0.0016 (Gli2) = 0.0054 (Atoh1)).