Fig. 5: Optimization for awake stroke: tMP stroke using permanent unilateral coil and magnet.

Animals underwent a short surgery (~25 min) under isoflurane (1.5%) for permanent placement of carotid coil and magnet (as outlined in methods), followed by injection of thrombin particles. a Instead of temporary ligation with nylon suture, animals received unilateral permanent placement of 0.18 mm Ø coil to the common carotid artery that is ipsilateral to cortex of intended injury and magnet placement (magnet left in place). di digastric muscle, scm sternocleidomastoid muscle, aa aortic arch, cc common carotid artery, t trachea. b 48-h infarct volume of tMPS compared to tMPS using coil both under anesthesia (tMPS n = 7, tMPS coil n = 15; unpaired two-sided t-test, p value). Forty-eight-hour corner and hanging wire behavior test between tMPS and tMPS using coil was not significant (corner test: tMPS n = 9, tMPS coil n = 13; hanging wire test: tMPS n = 11, tMPS coil n = 13; Mann–Whitney test). Data are presented as mean values ± SEM. c Animals were anesthetized (1.5% isoflurane) for surgery and LSI measurement. Animals were monitored for CBF as perfusion units (PU) following a baseline reading (BL), the unilateral coil placement on the carotid ipsilateral of permanent magnet placement (CC), following injection of particles and consequent tMP stroke (tMPS), 4 h following stroke, and up to 5 days. Sham animals followed identical surgical procedure and particle injection except for magnet placement (tMP). n = 4–5/group, unpaired two-sided t-test or Mann–Whitney test as appropriate. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.