Fig. 6: Effects of anesthesia and circadian cycle on tMP stroke.
From: A minimally invasive thrombotic model to study stroke in awake mice
a Coil and magnet placement surgeries were performed mid-morning and injected as one group (in order of surgeries) at zeitgeber times (ZT) 6-7 or at (ZT) 13-14 (anesthesia (ZT6-7) n = 10, awake (ZT6-7) n = 10, anesthesia (ZT13-14) n = 8, awake (ZT13-14) n = 10; 2-way ANOVA). b Representative cresyl violet staining shown of 48 h post awake daytime coil tMPS, (top) outlined in blue and 48 h post isoflurane anesthetized daytime coil tMPS (bottom) outlined in red. Infarct territory outlined in yellow. c Paired time-to-particle injection following coil and magnet surgery. Animals were injected in the order of surgery, completed within 1–2 h of full recovery (n = 6/group). d, Infarct volumes of mice shown as time from end of surgery to particle injection (day n = 7, night = 6; Kruskal–Wallis test shows no effect of time between surgery and stroke induction). Individual values and mean ± SEM are shown. Source data are provided as a Source Data file.
