Fig. 2: Ablation of endothelial MVP aggravates neointimal hyperplasia and atherosclerosis in mice. | Nature Communications

Fig. 2: Ablation of endothelial MVP aggravates neointimal hyperplasia and atherosclerosis in mice.

From: Endothelial major vault protein alleviates vascular remodeling via promoting Parkin-mediated mitophagy

Fig. 2

A Schematic illustration of the experimental design for injection of the tamoxifen and carotid artery ligation of male MvpECWT and MvpECKO mice. The figure is created using BioRender. Jiang B. (2025) https://BioRender.com/fazni0s. B Representative hematoxylin and eosin (H&E) and Elastic Van Gieson (EVG) staining images of non-ligated or ligated carotid arteries 4 weeks post ligation in MvpECWT and MvpECKO mice. Scale bar, 50 μm. C Neointima area, intima to media ratio, lumen area, media area, and vessel area of ligated carotid arteries in MvpECWT and MvpECKO mice were quantified (n = 10). D Experimental design for injection of the tamoxifen and WD feeding for 14 weeks in 6-week-old male MvpECWTApoe−/− and MvpECKOApoe−/− mice. The figure is created using BioRender. Jiang B. (2025) https://BioRender.com/ken5vk5. E Lipids profiles in MvpECWTApoe−/− and MvpECKOApoe−/− mice after 14 weeks of WD feeding (n = 8). F Representative images of en face Oil Red O staining of whole murine aortas. Quantification of the percentage of plaque area in whole aortas from MvpECWTApoe−/− (n = 7) and MvpECKOApoe−/− mice (n = 8). G Representative H&E staining images of cross-sections of murine aortic roots. Scale bars, 200 μm. Quantification of plaque area in aortic root (n = 8). H Representative Oil Red O staining images of cross-sections of the aortic root. Scale bars, 200 μm. Quantification of the percentage of Oil Red O staining of plaque area in whole aortic root plaque area (n = 8). I Representative Masson’s trichrome staining images of aortic root sections. Scale bars, 50 μm. Quantification of the percentage of collagen areas in plaques (n = 8). J Quantification of the percentage of necrotic core in plaques (n = 8). K Immunofluorescence staining images of CD68-positive macrophages in the aortic root. Scale bars, 50 μm. Quantification of CD68-positive staining of plaque area in aortic root (n = 8). L Immunofluorescence staining images of α-SMA-positive smooth muscle cells (SMCs) in the aortic root. Scale bars, 50 μm. Quantification of α-SMA positive staining of plaque area in aortic root (n = 8). Data are presented as mean ± SEM. Statistical significances are assessed by Mann-Whitney test (C, TG and TC in E) or two-tailed Student’s t-test (LDL-C and HDL-C in E–L). ns, no significance. Source data are provided as a Source Data file.

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