Fig. 1: Engineering Cas12a for multiplexed base editing.
From: Precision multiplexed base editing in human cells using Cas12a-derived base editors
A The ability of Cas12a to process gRNA arrays without accessory factors allows for the efficient delivery of many gRNAs into a single cell. Truncated gRNAs can be used to prevent deamination of neighboring bases. Created in BioRender. Schweitzer, A. (2025) https://BioRender.com/z2a6bsw. B Comparison of two published dLbCas12a-derived CBE and (C), one published dLbCas12a-derived ABE system for MBE in HEK293 cells. Heatmaps show normalized mean %T/%G values from three independent replicates (n = 3). Normalization was performed by subtracting the mean %T/%G values of the nt-ctrl condition from the mean %T/%G values of the experimental condition. Only positions 7–12 for CBEs and positions 8–12 of ABEs are shown, as those correspond to the editing window of the used systems. sg: single guide, dg: double guide, tg: triple guide. Source data are provided as a Source Data file.
