Fig. 4: Engineered gRNAs reduce frequencies of bystander mutations mediated by BEACON2.
From: Precision multiplexed base editing in human cells using Cas12a-derived base editors
A, B Editing outcomes of different gRNA designs for gRNA R1 and R7. C, D Sanger sequencing traces and %T values of a cell clone picked from the 15 nt wildtype sequence condition shown in (A and B). E Editing outcomes of six RUNX1 targeting gRNAs when expressed as either individual 20 nt or individual 15 nt gRNAs under the control of the CMV promoter. F, G Editing outcomes of the same six gRNAs when expressed as an array of 20 nt or 15 nt gRNAs. The order of gRNAs in array v2 in (G) is reversed from gRNA array v1 in (F). A, B, E–G show normalized mean %T values. Normalization was performed by subtracting the mean %T of the non-targeting (nt-ctrl) condition from the mean %T of the experimental condition. Source data are provided as a Source Data file.
