Fig. 5: Multiplexed base editing in diverse cell lines.
From: Precision multiplexed base editing in human cells using Cas12a-derived base editors
A Editing outcomes mediated by BEACON1 and BEACON2 when used with a triple gRNA array targeting RUNX1. B Editing outcomes mediated by BEACON1 and BEACON2 at 16 target sites in HeLa cells transfected with a CMV promoter-driven 16x gRNA array and the respective BE system. Editing outcomes in HEK293-B2 cells mediated by BEACON2 transfected with the same 16 × gRNA array as presented in Fig. 2F. A, B show normalized mean %T values at the highest edited C for each gRNA as determined based on the single gRNA condition. Normalization was performed by subtracting the mean %T of the non-targeting (nt-ctrl) condition from the mean %T of the experimental condition. Source data are provided as a Source Data file.
