Fig. 7: Postnatal SSRI exposure in wildtype pups and maternal SERT disruption lead to life-long changes in cortical encoding. | Nature Communications

Fig. 7: Postnatal SSRI exposure in wildtype pups and maternal SERT disruption lead to life-long changes in cortical encoding.

From: Perinatal serotonin signalling dynamically influences the development of cortical GABAergic circuits with consequences for lifelong sensory encoding

Fig. 7

a Sensory stimulation in adult mice, either SERT-WT/Het/KO or wild-types postnatally treated with sucrose/SSRI. b,c, GCaMP6s response to single-whisker stimulus in (b) SERT-WT/Het/KO or (c) sucrose/SSRI-treated mice. d,e, Violin plots of single-whisker stimulus response amplitude (d, Mann–Whitney U-test p = 0.004) and cell recruitment (e, t-test p = 0.032) in sucrose/SSRI-treated mice. GCaMP6s response to airpuff in (f) SERT-WT/Het/KO or (g) sucrose/SSRI-postnatally treated mice. Violin plots of airpuff response amplitude (h, t-test p = 0.046) and cell recruitment (i, t-test p = 0.007) in sucrose/SSRI-treated mice. j Confocal image of a S1BF cortical column (200 µm wide) on an adult WT cortex, illustrating DAPI (blue, left), VIP (cyan, centre left) and PV (magenta, centre right) distribution. k Distribution of PV (Two-way ANOVA: genotype p = 0.004, layer p < 0.001, genotype-layer interaction = 0.625), SST (Two-way ANOVA: genotype p = 0.934, layer p < 0.001, genotype-layer interaction = 0.971) and VIP (Two-way ANOVA: genotype p = 0.766, layer p < 0.001, genotype-layer interaction = 0.946) interneurons across layers for SERT-WT/Het/KO mice. l Distribution of Nkx2-1 (Two-way ANOVA: treatment p = 0.032, layer p < 0.001, treatment-layer interaction = 0.789), PV (Two-way ANOVA: treatment p = 0.322, layer p < 0.001, treatment-layer interaction = 0.903), SST (Two-way ANOVA: treatment p = 0.138, layer p = 0.01, treatment-layer interaction = 0.696) and VIP (Two-way ANOVA: treatment p = 0.032, layer p < 0.001, treatment-layer interaction = 0.691) interneurons across layers for sucrose/SSRI-treated mice. m Post-stimulus calcium responses to different stimuli were used for logistic regression analysis. n Heat map with the decoding accuracy of the different logistic regression classifiers; colours indicate accuracy minus threshold for significance of the classification as defined by bootstrapping. Positive values indicate significant classification accuracy. Sample sizes are 10 (WT), 8 (SERT-Het), 5 (SERT-KO), 6 (Sucrose) and 4 (SSRI). Traces are presented as mean ± SEM. Histology scatter plots are shown as individual animals (dots) plus the mean (line). Violin plots are presented as mean values ± max/min values.

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