Fig. 4: PVP activates AVB through ACR-15 and inhibits AVA through ACC-1/4.
From: Hierarchical competing inhibition circuits govern motor stability in C. elegans
a, b PVP-facilitated forward velocity was declined in acr-15(ok1214) mutants than in the wild type, and specific expression of ACR-15 in AVB reversed this decrease (n = 20 (wild type), 13 (acr-15), or 19 (AVB::ACR-15) animals) (p = 0.7531, 0.0057, 0.0339). c Left, Schematic diagram of the electrophysiological configuration. Right, representative depolarizing membrane potential in AVB by light activation of PVP in different genotypes. d Quantification of the depolarized membrane potential (Δ Voltage) in AVB, which was decreased in the acr-15(ok1214) mutant and rescued by the specific expression of ACR-15 in AVB (n = 9 (wild type), 6 (acr-15), or 5 (AVB::ACR-15) animals) (p = 0.938, 0.0276, 0.0309). e, f Increased backward movement caused by the inhibition of PVP was significantly attenuated in acc-1; acc-4 double mutants, and specific expression of ACC-1/ACC-4 in AVA rescued this attenuation (n = 33 (wild type, -ATR), 33 (wild type, +ATR), 22 (acc-1, -ATR), 20 (acc-1, +ATR), 29 (acc-4, -ATR), 30 (acc-4, +ATR), 18 (acc-1; acc-4, -ATR), 17 (acc-1; acc-4, +ATR), 21 (AVA::ACC-1/ACC-4, -ATR), 26 (AVA::ACC-1/ACC-4, +ATR) animals). Head gentle mechanical stimulation and optogenetic stimulation were implemented simultaneously (p = 0.9945, < 0.001, 0.0014, < 0.001, < 0.001, 0.0191, 0.9938, > 0.9999, 0.0231). g Left, Schematic diagram of the electrophysiological configuration used to record AVA by optical stimulation of PVP. Right, representative hyperpolarized membrane potential in AVA in the different genotypes. h PVP hyperpolarized membrane potential (Δ Voltage) in AVA was impaired in acc-1; acc-4 mutants and rescued by the specific expression of ACC-1/ACC-4 in AVA in AVA. (n = 7 (wild type), 5 (acc-1; acc-4), or 5 (AVA::ACC-1/ACC-4) animals) (p = 0.7027, < 0.001, < 0.001). The above p-values are all listed in the order of significance symbols (from top to bottom, left to right). Black arrows, gentle touching of the head. Yellow arrows, light stimulation. Black bars, light off. Yellow or blue bars, light on. Yellow or blue shaded, light stimulation. Animals #, the animal number used in the experiments. Unless otherwise stated, *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant according to one-way ANOVA. Error bars, SEM. Source data are provided as a Source Data file.
