Fig. 1: Augmented CgA protein levels in AD and CBD patient samples and PS19 transgenic mice. | Nature Communications

Fig. 1: Augmented CgA protein levels in AD and CBD patient samples and PS19 transgenic mice.

From: Chromogranin A deficiency attenuates tauopathy by altering epinephrine–alpha-adrenergic receptor signaling in PS19 mice

Fig. 1

A Representative Western blot (WB) of postmortem frontal cortex extracts showing levels of CgA, p-Tau, total Tau and Actin in six Braak stage 6 (Brk 6) and Braak stage 0-2 (Brk 0-2) postmortem patient frontal cortex lysates. B Representative WB of hippocampus extracts showing levels of CgA, p-Tau, total Tau and Actin in Brk6 and Brk (0-1) hippocampus lysates. C Quantification of WB in (A) (Brk 0-2, n = 12; Brk6, n = 14) (t = 3.19, df = 22.32). D Quantification of WB in (B) (Brk 0-2, n = 6; Brk6, n = 6) (t = 2.665, df = 9.49). E Representative immunofluorescence (IF) images showing CgA signal in the hippocampus of Brk 6 and Brk 1-2 samples. Scale bar = 20 μm. F ImageJ quantification of the immunoreactive CgA in the hippocampus shown as area fraction (%) of total hippocampus (n = 8 per group) (t = 3.853, df = 12.15). G WB of frontal cortex lysates of CBD and normal control patients showing levels of CgA, pTau, Tau and actin. H Quantification of CgA levels in WB shown in (G), normalized to actin levels (n = 7 per group) (t = 2.687, df = 9.028). I WB of frontal cortex lysates of WT (n = 6) and PS19 (n = 6) mice for CgA, Tau and actin. J Quantification of WB shown in I for CgA levels, normalized to actin levels (n = 6 per group) (t = 2.728, df = 9.455). K Schematic (Created in BioRender81) showing the experimental plan of CgA treatment in neuron and representative image of MAP2 and MC1 staining after CgA treatment. Scale bar = 200 µm. L Quantification of MAP2 and MC1 staining after CgA treatment. (t = 6.623, df = 7.284). M Schematic of organotypic slice culture (OTSC) generation, treatment and imaging (Created in BioRender82). Representative IF images using MC1 antibody showing misfolded Tau species in organotypic hippocampal slices from WT (top) and CgA-KO (bottom) mice transduced with AAV tau P301S and treated with K18 fibrils. Scale bar = 200 μm. N ImageJ quantification of MC1 area fraction from images as represented in (M) (n = 6), t = 2.49, df = 8.635. P-values were calculated using unpaired two-tailed T-test with Welch’s correction. Data are presented as mean values +/− SEM. Source data are provided as a Source Data file.

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