Fig. 3: Ablation of CgA improved spatial learning and memory, and extended the life span of PS19 mice. | Nature Communications

Fig. 3: Ablation of CgA improved spatial learning and memory, and extended the life span of PS19 mice.

From: Chromogranin A deficiency attenuates tauopathy by altering epinephrine–alpha-adrenergic receptor signaling in PS19 mice

Fig. 3

A Distance traveled each day to reach the platform during seven days training trials of the Morris Water-Maze Test. CgA-KO/PS19 (n = 20), CgA-KO (n = 16), PS19 (n = 19) and WT (n = 18). p-value was calculated using Two-way ANOVA (F18, 420 = 3.013, P < 0.0001) Tukey’s Multiple comparison test. B Area Under the Curve (AUC) for the distance traveled during the training period. P-value was calculated using One-way ANOVA (F3, 70 = 43.76, P < 0.0001) Dunnett’s Multiple comparison test. C Probe trial performed 24 hr after the 7th day of training for all the mice. The swimming paths in the probe trial of representative mice of all four groups are shown (the square marks the location of the platform during the goal acquisition trials). D Time taken (latency) to reach the target platform. CgA-KO/PS19 (n = 20), CgA-KO (n = 16), PS19 (n = 19) and WT (n = 18). P-value was calculated using One-way ANOVA (F3, 69 = 16.14, P < 0.0001) Sidak’s Multiple comparison test. E Number of entries in the zone where the platform was present (correct zone). CgA-KO/PS19 (n = 20), CgA-KO (n = 16), PS19 (n = 19) and WT (n = 18). P-value was calculated using One-way ANOVA (F3, 69 = 11.5, P < 0.0001) Sidak’s Multiple comparison test. F Swimming speed of all the mice during the probe trial day. CgA-KO/PS19 (n = 20), CgA-KO (n = 16), PS19 (n = 20) and WT (n = 18). P-value was calculated using One-way ANOVA (F3, 70 = 4.99, P = 0.0034) Sidak’s Multiple comparison test. G Novel Object Recognition scores of CgA-KO/PS19 and PS19 mice. [WT (n = 15), CgA-KO (n = 16), PS19 (n = 19) and CgA-KO/PS19 (n = 20)]. P-value was calculated using Kruskal-Wallis test for NOR. H Time before falling in the Rotarod test for all four mice groups [WT (n = 18), CgA-KO (n = 16), PS19 (n = 20) and CgA-KO/PS19 (n = 20)]. P-value was calculated using Two-way ANOVA (F18, 420 = 1.328, P = 0.1656) Turkey’s Multiple comparison test. I Longevity of PS19 and CgA-KO/PS19 mice shown as a Kaplan-Meier plots (n = 20 per group). P-value was calculated using Gehan-Breslow-Wilcoxon test (Chi square = 23.86, df = 1). Data are presented as mean values +/− SEM. Source data are provided as a Source Data file.

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