Fig. 2: The clb2-pp mutation causes a mitotic delay.
From: Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing
a Single-cell measurements of time from SPB separation to spindle elongation in asynchronous wild-type (WT) or clb2-pp cells carrying mCherry-labeled Spc42. Each data point represents a single cell, and the bars indicate median ± SD. The sample size (n) is noted at right. See Supplementary Fig. 2a for representative images. b Western blots of Myc-tagged securin after release from a G1 arrest in the indicated yeast strains. Alpha factor was added back at the 60 min time point. GAPDH loading control on the lower blot. Representative of 10 independent experiments. c Western blots of Myc-tagged Ndd1 after release from a G1 arrest in the indicated yeast strains. These strains carry a second copy of Ndd1-Myc under the control of a GAL promoter, and experiments were performed in galactose media. Alpha factor was added back at the 60 min time point. GAPDH loading control on the lower blot. Representative of 2 independent experiments. d Same as (b) with swe1∆. Representative of 2 independent experiments. e Same as (b) with mad2∆. Representative of 2 independent experiments. f Same as (b) with apc1-∆loop. Representative of 2 independent experiments. Source data are provided as a Source Data file.
