Fig. 5: Phospho-pocket mutations reduce phosphorylation of polarisome subunits.
From: Phosphate-binding pocket on cyclin B governs CDK substrate phosphorylation and mitotic timing
2.5 μM of purified Bud6 fragment (aa 126–360) or 5 μM of purified Spa2 fragment (aa 524–670) was incubated with 100 nM wild-type or clb2-pp Clb2-Cdk1 plus wild-type or mutant Cks1 and radiolabeled ATP. Diagrams at top indicate optimal (S/T*-P-x-K/R; green) and suboptimal (S/T*-P; yellow) CDK consensus sites (S: circle; T: triangle) in the tested fragment (see Supplementary Fig. 4 for complete sequences). Representative of 3 independent experiments. Coomassie Blue-stained gels in Supplementary Fig. 8. Source data are provided as a Source Data file.
