Fig. 5: Co-inhibition of DNA-PK and Polϴ increases precision of dual pegRNA editing systems TwinPE, HOPE, Bi-PE, and TwinPEn.
From: Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems
a Schematic representation of TwinPE-mediated replacement with possible editing outcomes. b TwinPE mediated attP (50 bp) or attB site (38 bp) installation at three different sites (AAVS1, ACTB, CCR5) with indicated drug treatments in HEK293T cells. c Schematic representation of HOPE and Bi-PE editing. d Editing outcomes of HOPE (insertion) and Bi-PE2 and Bi-PE3 (five individual substitutions) at indicated target site subjected to indicated drug treatments in HEK293T cells. e Schematic representation of TwinPEn-mediated replacement and possible editing outcomes. f TwinPEn mediated attP (50 bp) or attB site (38 bp) installation at three different sites (AAVS1, ACTB, CCR5) with indicated drug treatments in HEK293T cells. All amplicon sequencing data described above was analyzed using CRISPResso2 in HDR mode. All bar plots show mean ± SD of n = 3 biological replicates. Drug treatments: 1i = DNA-PKi; 2+i = DNA-PKi + PolQi1 + PolQi2. Source data are provided as a Source Data file. RTT Reverse-Transcriptase Template, bp base pairs, del. deletion, ins. insertion.
