Fig. 2: Abdominal condition of adult female mosquitoes injected with Ringer’s solution. | Nature Communications

Fig. 2: Abdominal condition of adult female mosquitoes injected with Ringer’s solution.

From: 3,4-Dihydroxyphenylacetaldehyde synthase evolved an ordered structure to deliver oxygen to pyridoxal 5’-phosphate for cuticle assembly in the mosquito Aedes aegypti

Fig. 2

a–c Treated mosquitoes that survive 72 h after laying eggs were then injected with 5 µL Ringer’s solution. a Surviving mosquito first treated with DEPC water, and then injected with Ringer's solution. b Surviving mosquito treated with gus‑dsRNA, and then injected with Ringer's solution. c Surviving mosquito treated with AeDHPAAS‑dsRNA, and then injected with Ringer's solution. d The percentage of mosquitoes from each group with abdominal rupturing. Thirty female adults were analyzed from each group, and the experiment was replicated three times. Data were presented as mean values ± SD. Ordinary one-way ANOVA with two-sided Tukey’s multiple comparisons test was used for statistical analysis. DEPC 95% CI = -7.42–17.42; GUS 95% CI = -2.45–12.45; DS 95% CI = 12.58–37.42. DEPC vs GUS, Q = 0.00, df = 6, P > 0.99; DEPC vs DS, Q = 7.81, df = 6, P < 0.01. ** indicates P < 0.01; ns indicates no significant difference. Source data are provided as a Source Data file. DEPC mosquitoes treated with DEPC water, GUS mosquitoes treated with gus‑dsRNA, DS mosquitoes treated with AeDHPAAS‑dsRNA.

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