Fig. 8: Transcriptional trajectory analysis of GFP-positive cells. | Nature Communications

Fig. 8: Transcriptional trajectory analysis of GFP-positive cells.

From: Auricular malformations are driven by copy number variations in a hierarchical enhancer cluster and a dominant enhancer recapitulates human pathogenesis

Fig. 8

a UMAP dimensional reduction visualizes Hmx1+ expressed cells combined across E10.5, E12.5, and E14.5. CNCCs-related fibroblasts clusters are outlined with dotted lines for further analysis in b and c. b A dot plot displays two marker genes for each cluster identified within the CNCCs-related fibroblasts. The cell clusters of 1-7 are the same as in a. c Developmental trajectory analysis from E10.5 CNCC mesenchymal cells (cluster 6) to the fibroblasts defined in (a) is illustrated. The cell clusters of 1-7 are the same as in (b). d The expression of cartilage, fibroblast, muscle, and epidermis development-related downregulated DEGs across nine cell clusters (including 7 fibroblast, chondrocyte, and myogenic cell clusters) is shown. The heatmap on the left panel depicts the fold change of each gene. e, f In situ hybridization staining for Hmx1 and Dmkn expression in wild-type and mEC1dup/dup embryos at upper (e) and lower (f) regions of the pinna. Scale bars measure 100 µm. Three independent replicates were performed.

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