Fig. 5: Proposed model of the mechanism of MCT8-mediated thyroxine (T4) transport and of its inhibition.
From: Molecular mechanism of thyroxine transport by monocarboxylate transporters
Schematic illustration of MCT8 conformations during the thyroid hormone transport cycle. The states marked with * represent experimentally obtained conformations in this study, while ** were inferred by homology modeling and supported by our assays. The T4-bound IFS cartoon was derived from the structure of T4-bound MCT10, determined in this work. MCT8 D424N binds but does not transport T4, as shown by in vitro binding and in cellulo T4 uptake assays. To derive the position of residues in the long isoform of MCT8, 74 amino acids should be added (Supplementary Fig. 10).
