Fig. 2: Identification of AHAS for condensing branched-chain aldehydes with pyruvate.
From: Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism
a Representative GC-FID chromatogram results of the carboligation of isobutyraldehyde (IBAL) and pyruvate catalyzed by MR-IlvBN, MR-AlsS, and MR-Ilv2c. Compound 1a represents substrate IBAL, 1b represents product 3-H-4-MP-2-one, 1c represents 4-M-PDO. b Representative GC-MS spectrum to validate the chemical structure of compound 1c. Red spectrum represents 1c produced by the biocatalytic system; blue spectrum represents 4-M-PDO from NIST library. c Representative GC-FID chromatogram results of the carboligation of isovaleraldehyde and pyruvate catalyzed by MR-Ilv2c. d Representative GC-FID chromatogram results of the carboligation of 2-methylbutyraldehyde and pyruvate catalyzed by MR-Ilv2c. Experiments were carried out in three biological repeats, and only representative results are shown.
