Fig. 4: De novo synthesis of diols via the recursive carboligation cycle from BCAA metabolism.
From: Microbial synthesis of branched-chain β,γ-diols from amino acid metabolism
a The dual-plasmid system was transformed into E. coli RARE to evaluate the recursive carboligation cycle for synthesizing diols from glucose. The biosynthetic route for diol synthesis is shown in the dotted box. b The distribution of L-valine pathway derivatives of 4-M-PDO and its precursors. c The distribution of L-leucine pathway derivatives of 5-M-HDO and its precursors. d The distribution of L-isoleucine pathway derivatives of 4-M-HDO and its precursors. e The percentage profile of diols produced by engineered strain MR-01. The average and standard deviation are obtained from three biological replicates. Source data are provided as a Source Data file.
