Fig. 3: Cotadutide induces changes in the hepatic proteome of metabolism-related proteins in DIO mice. | Nature Communications

Fig. 3: Cotadutide induces changes in the hepatic proteome of metabolism-related proteins in DIO mice.

From: GLP-1R/GCGR dual agonism dissipates hepatic steatosis to restore insulin sensitivity and rescue pancreatic β-cell function in obese male mice

Fig. 3

Experimental design for proteomic and phosphoproteomics study in which DIO mice were treated with cotadutide or vehicle for 28 days, then injected with PBS or insulin yielding four groups: vehicle + PBS (VP), vehicle + insulin (VI), cotadutide + PBS (CP), and cotadutide + insulin (CI). Created in BioRender. Kajani, S. (2025) https://BioRender.com/t03r158 (A). Volcano plot of significant up- (red, 531) or down-regulated (blue, 431) hepatic proteins comparing CP/VP as identified via proteomics (B). KEGG pathway analysis of all significantly differentially regulated hepatic proteins comparing CP/VP (C). n = 4 mice per group. In (B), dashed line denotes p = 0.05 and solid lines mark cut-off values for abundance ratio <0.76 or >1.315. The in-built one-way ANOVA statistical test in Proteome Discover (PD) was used to generate p values. In (C), KEGG term ranking based on FDR and significance based on FDR <0.05. Source data are provided as a Source Data file.

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