Fig. 5: Gfi1 is necessary for the accumulation of terminally differentiated Tim-3hi tumor infiltrating CD8+ T cells in late-stage tumors.
From: Gfi1 controls the formation of effector-like CD8+ T cells during chronic infection and cancer
A Tumor growth dynamics of Gfi1tdTomato reporter mice subcutaneously inoculated with 1 × 105 MB49 cells. B Identification of TCF1hi progenitor and Tim-3hi effector CD8+ TILs with total cell counts and ratios of Tim-3hi to TCF1hi on Days 7 (n = 11), 14 (n = 13), and 22 (n = 11) post-inoculation. C Histograms showing the expression of 2B4, LAG-3, PD-1, and TOX in TCF1hi (progenitor) and Tim-3hi TILs. D Flow plots of Gfi1tdTomato expression in progenitor and Tim-3hi CD8+ TILs at different time points, with the frequencies for subsets on Day 14 shown in the scatter dot plot (n = 6). E Flow plots showing expression of Gfi1tdTomato with TCF1, TOX, Eomes, and T-bet in progenitor cells with their GMFIs depicted (n = 5–7). F Tumor growth dynamics and masses on Days 7, 12, and 21 in WT (n = 10, 16, & 17) and Gfi1cKO (n = 11, 16 & 18) mice post the inoculation of MB49 cells. G Flow plots showing the abundance of Tim-3hi and TCF1hi progenitor TILs in tumors from WT (n = 10, 18, & 17) and Gfi1cKO (n = 10, 16, & 18) mice as well as their ratios on Day 7, 12, and 21 post-tumor inoculation. H Distribution of Tim-3hiT-bethi and Tim-3hiT-betlo effector TILs as well as Tim-3loT-betint progenitor TILs (Left) and their frequencies of TOXhi and Eomeshi cells (Right) on Day 21 tumors from WT (n = 8) and Gfi1cKO (n = 10) mice. I Perforin and granzyme (B) production by WT (n = 6) and Gfi1cKO (n = 7) CD8+ TILs. Data (mean ± s.e.m.) were pooled results from 2–4 independent experiments, with each dot indicating an individual mouse. Data were analyzed by two-way ANOVA (B Left, D, F–H), one-way ANOVA (B, Right), repeated one-way ANOVA (E), and unpaired two-tail t test (I). Holm-Sidak’s post hoc test was used unless otherwise stated. Source data are provided as a Source Data file.
