Fig. 10: TCR signaling is not involved with TIGIT-mediated T-cell suppression. | Nature Communications

Fig. 10: TCR signaling is not involved with TIGIT-mediated T-cell suppression.

From: TIGIT deficiency promotes autoreactive CD4+ T-cell responses through a metabolic‒epigenetic mechanism in autoimmune myositis

Fig. 10

a CD4+ T cells isolated from Tigit+/+ or Tigit−/− mice were stimulated with αCD3/CD28 for 3 d. IKKα, IKKβ, p-IκBα, IκBα, p-p65, and p65 expression were quantified by western blot. Data from six biologically independent replicates. b, c CD4+ T cells from Tigit+/+ or Tigit−/− mice were stimulated with PMA + PHA or OKT3 + PMA for 48 h. CD44, CD69, and CD25 expression was measured by flow cytometry. Data from five biologically independent replicates. d, e CD4+ T cells isolated from Tigit+/+ or Tigit−/− mice were labeled with CFSE and stimulated with PMA + PHA or OKT3 + PMA for 3 d. T-cell proliferation was measured by flow cytometry. Data from six biologically independent replicates. f–j CD4+ T cells from Tigit+/+ or Tigit−/− mice were stimulated with anti-CD3 alone (αCD3) or anti-CD3 plus anti-CD28 (αCD3/CD28) for 3 d. f–h CD4+ T cells were labeled with CFSE, and cell proliferation was assessed by flow cytometry. I, j IL-2 expression was assessed by flow cytometry. Representative histograms were shown. Data from five biologically independent replicates. All data are mean ± SEM. Statistics were done by two-way ANOVA followed by adjustments for multiple comparisons.

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