Fig. 7: Loss of epithelial integrity is prevented by heat inactivation of mechanically or antibacterially lysed P. aeruginosa cultures. | Nature Communications

Fig. 7: Loss of epithelial integrity is prevented by heat inactivation of mechanically or antibacterially lysed P. aeruginosa cultures.

From: Adjunctive phage therapy improves antibiotic treatment of ventilator-associated-pneumonia with Pseudomonas aeruginosa

Fig. 7

Measuring the transepithelial electric resistance (TEER) of epithelial cell monolayers by using ECISTM system upon exposure to supernatants of a mechanical lysed bacteria via ultrasonication (Branson Sonifier 450, 400 W Titan-MicroTip) or b heat-inactivated (HI) supernatants of mechanically lysed or antibacterial treated PAO1-liquid cultures (120 min time point, see Fig. 5). Heat-inactivation was done at 60 °C for 60 min. Untreated Control are only HPAEpiC, same dataset in each figure. Means of normalized resistance (900 Hz) versus time curves are shown; n = 3. ECIS electric cell-substrate impedance sensing, HPAEpiC human primary alveolar epithelial cells, MIC minimum inhibitory concentration. Source data are provided as a Source Data file.

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