Fig. 8: Phage treatment improved lung epithelial cell integrity during P. aeruginosa infection in vitro. | Nature Communications

Fig. 8: Phage treatment improved lung epithelial cell integrity during P. aeruginosa infection in vitro.

From: Adjunctive phage therapy improves antibiotic treatment of ventilator-associated-pneumonia with Pseudomonas aeruginosa

Fig. 8

Measuring the transepithelial electric resistance (TEER) of epithelial cell monolayers by using ECISTM system upon infection with PAO1 (MOI 1) and subsequent treatment with a meropenem (Mero; MIC = 1 µg/mL), b the Pseudomonas-specific phage cocktail (Phages), or c a combination of both in indicated concentrations for 72 h. d Direct comparison of adjunctive phage therapy and single therapy. Means of normalized resistance (4000 Hz) versus time curves are shown; each isolate was run in 3 independent experiments while each condition where run in triplicates (96W10idf). Dotted line indicates the starting time of starving (−2 h), infection (0 h), and treatment (2 h). Uninfected Control and Infection Control (PAO1 MOI 1) same dataset in each figure. Uninfected Control are only HPAEpiC. Color coding for groups: Uninfected control group: black, Infected control group: red, Meropenem group: shades of green, Phages group: shades of blue; Combination group: shades of orange. ECIS electric cell-substrate impedance sensing, HPAEpiC human primary alveolar epithelial cells, MIC minimum inhibitory concentration. Source data are provided as a Source Data file.

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