Fig. 10: Proximity analysis of Th cells within 50 µm of each ILC subtype in appendix, ileum, lymph node, tonsil, and follicular lymphoma (n = 8 independent biological samples for each tissue type, 1 mIF staining batch per tissue type).

Schematic overview of the proximity analysis data for appendix, ileum, lymph node, tonsil, and follicular lymphoma. Each circular diagram represents the proximity of each “small” cell (Th2 cells in A, Th1 and Th17 cells in B) within a 50 µm radius (2 × 25 µm concentric) of the center of the target cell nucleus, drawn in the middle (ILC1, ILC2, and ILC3). The mean number and location of each “small” cell are shown schematically within the 2 × 25 µm radius proximity of the target cells (ILC1, ILC2, and ILC3). The small pie charts represent the percentages of each “small” cell population (Th cells) within (dark grey pie part) and over (light grey pie part) the 50 µm radius of the target cells (ILC1, ILC2, and ILC3). Only one circular diagram is shown in (B) because the number and location pattern of Th1 and Th17 cells is the same for all the tissue types analyzed, whereas each diagram for each ILC subtype is divided by 5 (for appendix, ileum, lymph node, tonsil and follicular lymphoma) in (A) to display per quadrant the proximity analysis data overview that are different for each tissue type. C Heatmaps show the percentage of Th1, Th2 or Th17 (red gradient) according to the ILC1, ILC2, or ILC3 distance (blue gradient; 5 µm steps) in tonsil, lymph node, and follicular lymphoma, appendix and ileum. Bars show the percentage of Th cells within 50 µm of an ILC1, ILC2, or ILC3 in tonsil, lymph node, follicular lymphoma, appendix, and ileum. Source data are provided as a Source Data file. Partially created in BioRender. Bezombes, C. (2025) https://BioRender.com/shbs5qf.