Fig. 2: Cluster analysis reveals distinct ILC and T helper subset partnership. | Nature Communications

Fig. 2: Cluster analysis reveals distinct ILC and T helper subset partnership.

From: Spatial mapping of innate lymphoid cells in human lymphoid tissues and lymphoma at single-cell resolution

Fig. 2

An important IL-7Rαpool resides within lymphoid organs and ILC2 are the main ILC-subtype penetrating lymphoid follicles (FZ). A A Standardized cell densities clustermap of ILCs and T helper cells in relation to patient characteristics (gender and age range) and tissue types (tonsil, appendix, ileum, lymph node, spleen, and thymus) (n = 8 independent biological samples per lymphoid tissue type). Four clusters as determined using the elbow method and the K-means algorithm: Blue, Green, Beige, Deep red. B B, T, IL-7Rαpool, and ILCs quantification in lymphoid organs. Split-violin plots representing B, T and IL-7Rαpool cell densities (number/mm2) inside (left) and outside (right) of the follicular zone of lymph node, tonsil, appendix and ileum, in the white (left) and red (right) pulp of spleen, and in the medulla (left) and cortex (right) of thymus (n = 8 independent biological samples per lymphoid tissue type). C Split-violin plots representing the percentage of ILC phenotypes (per IL-7Rαpool) inside (left) and outside (right) of the follicular zone of lymph node, tonsil, appendix and ileum, in the white (left) and red (right) pulp of spleen, and in the medulla (left) and cortex (right) of thymus. D–G Classic mIF of tonsil for CD20 (aqua), TCRαδ (green), and IL-7Rα (red). Interface between follicular and extra-follicular zones: dashed line. Scale bar: 100 µm. Exemple of ILC1 (CD20− TCRαδ− IL-7Rα+ TBX21+ EOMES±) (H), ILC2 (CD20− TCRαδ− IL-7Rα+ GATA-3+ ICOS+) (I), and ILC3 (CD20− TCRαδ− IL-7Rα+ RORγT+ AHR+) (J) identified cells. Scale bar: 10 µm. Box-plots within Violin Plots, created using Seaborn in Python, present the median value (white dot, 50th percentile) in between the first quartile (the middle value between “minimum non-outlier” and median (marked as Q1, portrays the 25th percentile) and third quartile (the middle value between “maximum non-outlier” and median (marked as Q3, portrays the 75th percentile)). Source data is provided as a Source Data file.

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