Fig. 6: ILC1 and ILC3 are the dominant ILC-populations in intestinal mucosal tissue, where they are colocalizing with Th2.

Topographic distribution of NK/ILC1ie (dark blue), ILC1 (orange), ILC2 (light blue), and ILC3 (pink) in appendix (A) and ileum (B, C) (n = 8 independent biological samples for each tissue type, 1 mIF staining batch per tissue type). A H&E overview of appendix with corresponding ILC distribution map (detailed in caption). B cells (light grey) are used to highlight the follicular areas. Scale bar: 2 mm. B H&E overview of ileum with corresponding ILC distribution map (detailed in caption). B cells (light grey) are used to highlight the follicular areas. Scale bar: 2 mm. C Classic mIF staining for CD20 (aqua), TCRαδ (green), EOMES (orange), TBX21 (red), and IL-7Rα (pink) in ileum. DAPI nuclear counterstain (blue). Dashed line and hashtag indicating the follicular zone. ILC1s indicated by white arrows. Scale bar: 200 µm. D Split-violin plots representing the ILC phenotype densities (number/mm²) in the follicular (left) and extra-follicular (right) zones of the appendix and ileum (classic mIF using CD20). E Infiltration analysis plot with interface (InF) between extra-follicular zone (OUT) and follicular zone (IN) for IL-7Rα⁺ ILC1 (orange), ILC2 (light blue) and ILC3 (pink) in appendix (upper) and ileum (lower). Box-plots within Violin Plots, created using Seaborn in Python, present the median value (white dot, 50th percentile) in between the first quartile (the middle value between “minimum non-outlier” and median (marked as Q1, portrays the 25th percentile) and third quartile (the middle value between “maximum non-outlier” and median (marked as Q3, portrays the 75th percentile)). Source data are provided as a Source Data file.