Fig. 7: ILC2 and Th2 dominate the lymph node and tonsil. | Nature Communications

Fig. 7: ILC2 and Th2 dominate the lymph node and tonsil.

From: Spatial mapping of innate lymphoid cells in human lymphoid tissues and lymphoma at single-cell resolution

Fig. 7

Topographic distribution of NK/ILC1ie (dark blue), ILC1 (orange), ILC2 (light blue) and ILC3 (pink) in lymph node (A) and tonsil (B, C) (n = 8 independent biological samples for each tissue type, 1 mIF staining batch per tissue type). A H&E overview of lymph node with corresponding ILC distribution map (detailed in the caption). B cells (light grey) are used to highlight the follicular areas. Scale bar: 1 mm. B H&E overview of tonsil with corresponding ILC distribution map (detailed in caption). B cells (light grey) are used to highlight the follicular areas. Scale bar: 2 mm. C Classic mIF staining for CD20 (aqua), TCRαδ (green), GATA-3 (orange), ICOS (red), and IL-7Rα (pink) in tonsil. DAPI nuclear counterstain (blue). Hashtag indicating the germinal center. ILC2 indicated by white arrows. Scale bar: 100 µm. D Split-violin plots representing the ILC phenotype densities (number/mm2) in the follicular (left) and extra-follicular (right) zones of the lymph node and tonsil (classic mIF using CD20). E Infiltration analysis plot with interface (InF) between extra-follicular zone (OUT) and follicular zone (IN) for IL-7Rα+ ILC1 (orange), ILC2 (light blue), and ILC3 (pink) in lymph node (upper) and tonsil (lower). Box-plots within Violin Plots, created using Seaborn in Python, present the median value (white dot, 50th percentile) in between the first quartile (the middle value between “minimum non-outlier” and median (marked as Q1, portrays the 25th percentile) and third quartile (the middle value between “maximum non-outlier” and median (marked as Q3, portrays the 75th percentile)). Source data is provided as a Source Data file.

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