Fig. 8: Loss of IL-7Rαpool and ILC subtypes, and altered T helper cell subsets in follicular lymphoma.
Topographic distribution of NK/ILC1ie (dark blue), ILC1 (orange), ILC2 (light blue), and ILC3 (pink) in follicular lymphoma (FL) (n = 8 independent biological samples, 1 mIF staining batch). A H&E overview of FL with corresponding ILC distribution map (detailed in caption). B cells (light grey) are used to highlight the follicular areas. Scale bar: 2 mm. B Infiltration analysis plot with interface (InF) between extra-follicular regions (OUT) and follicular regions (IN) for IL-7Rα+ ILC1 (orange), ILC2 (light blue) and ILC3 (pink) in FL. C Classic mIF staining for CD20 (aqua), TCRαδ (green), EOMES (orange), TBX21 (red), and IL-7Rα (pink) showing ILC1s (white arrows) in follicular regions (dashed line and hashtag) and outside the follicles. DAPI nuclear counterstain (blue). Hashtag indicating the follicles. Scale bar: 100 µm. D Same as “C” but for classic mIF staining using GATA-3 (orange) and ICOS (red) (instead of EOMES and TBX21) and showing the spatial distribution of ILC2 around the follicle interface (dashed line). Scale bar: 100 µm. E Split-violin plots (left panel) representing the ILC and T helper densities (number/mm2) in the follicular (left) and extra-follicular (right) zones of the FL. Bubble plots (right panel) comparing the overall relative cell densities (bubble size) and the spatial distribution of each ILCs with their T helper counterparts around the follicular interfaces in the lymph node, tonsil, and FL. X-axis: mean cell density % inside the interface (log10 scale). Y-axis: mean cell density % outside the interface. LN: Lymph Node (yellow). TS Tonsil (green). FL Follicular Lymphoma (orange). Box-plots within Violin Plots, created using Seaborn in Python, present the median value (white dot, 50th percentile) in between the first quartile (the middle value between “minimum non-outlier” and median (marked as Q1, portrays the 25th percentile) and third quartile (the middle value between “maximum non-outlier” and median (marked as Q3, portrays the 75th percentile)). Source data are provided as a Source Data file.
