Fig. 2: Generation of the FS mouse model Q84Pfs and its anatomical brain deficits. | Nature Communications

Fig. 2: Generation of the FS mouse model Q84Pfs and its anatomical brain deficits.

From: The patient-specific mouse model with Foxg1 frameshift mutation provides insights into the pathophysiology of FOXG1 syndrome

Fig. 2

a Schematics of mouse FOXG1 full-length (FOXG1-fl) protein and Q84Pfs fragment, and the antigenic regions for two FOXG1 antibodies. The antibody FOXG1-N-Ab detects both FOXG1-fl and Q84Pfs proteins. The antibody FOXG1-C-Ab recognizes FOXG1-fl, but not Q84Pfs. b Sanger sequencing results of the Foxg1 gene of wild-type (WT), Q84Pfs-Het, and Q84Pfs-Homo mice. An extra cytosine (C) insertion occurred after the 250th nucleotide cytosine in the Q84Pfs allele, indicated as a cytosine duplication (dupC) in the sequencing data of Q84Pfs-Homo mice. c The morphology of WT, Q84Pfs-Het, and Q84Pfs-Homo brains at E18.5. d The representative images for Nissl stain of the serial coronal sections of WT and Q84Pfs-Het adult brains. The anterior-to-posterior sections were as shown. e Magnified views of the midline areas as marked as red-dotted rectangles in (d). Yellow and red arrows indicate the septum and corpus callosum deficits, respectively. f Magnified views of the hippocampus as marked as blue-dotted rectangles in (d). g Quantification of the corpus callosum (CC) thickness in the serial coronal sections of WT and Q84Pfs-Het adult brains from the anterior (1:**p = 0.0079, 2:p = 0.4127, 3:p = 0.5556, 4:p = 0.7302, 5:p = 0.3333), corresponding to the area of the most left column of (e), to the posterior, corresponding to the area of the most right column of (e). h, i Quantification of the length of the dentate gyrus (DG, ****p < 0.0001) and the size of the hippocampus (HP, *p = 0.0107) areas in the serial coronal sections of WT and Q84Pfs-Het adult brains. (g–i, n = 4 for WT, 5 for Q84Pfs-Het mice). Scale bars, 1 mm (d) or 200 μm (e, f). j–n Quantification of body weight (j, p = 0.7432), brain weight (k, ****p < 0.0001), anterior-posterior length (m, ****p < 0.0001), or the total area (n, ****p < 0.0001) of the corpus callosum in the sagittal section of the brains, as depicted in (l) in WT and Q84Pfs-Het adult mice. (j, k, m, n, n = 7 males, 9 females for WT, 8 males and 7 females for Q84Pfs-Het mice). The error bars (g–k, m, n) represent SEM. Two-tailed Mann-Whitney test (g, m, n) or unpaired two-tailed t test (h–k). Only the representative images are shown.

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