Fig. 4: ACT-DC induces a systemic immune response with memory. | Nature Communications

Fig. 4: ACT-DC induces a systemic immune response with memory.

From: Integrating antigen capturing nanoparticles and type 1 conventional dendritic cell therapy for in situ cancer immunization

Fig. 4

a Schedule of the study to profile immune cells in tDLNs and spleen. Created in BioRender. Zhao, Z. (2025) https://BioRender.com/3ow34vf. b–g Number of different immune cells in the tDLN of mice treated with ACT-DC or control therapies (n = 5 biologically independent animals per group) including macrophages (b), cDC1 (c), cDC2 (d), CD8 and CD4 T cells (e), Adpgk tetramer-positive CD8 T cells (f), and IFN-γ expressing CD4 and CD8 T cells (g). h–j Number of memory CD8 T cells in the tDLN and spleen of mice receiving the ACT-DC or control therapies (n = 5 biologically independent animals per group). h Representative flow cytometry plots showing the memory CD8 T cells in the tDLN. i Total number of effector memory (CD44 + CD62L−) and central memory (CD44 + CD62L+) CD8 T cells in the tDLN. j Total number of memory CD8 T cells in the spleen. For b–g, i, j, data were presented as mean values ± SEM. Statistical analysis for (b–g, i, j): one-way ANOVA followed by Dunnett test. Source data are provided as a Source Data file.

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