Fig. 5: Activation of the VP-LC Glu projection enhances brain arousal at different movement states. | Nature Communications

Fig. 5: Activation of the VP-LC Glu projection enhances brain arousal at different movement states.

From: A ventral pallidum-locus coeruleus-lateral hypothalamus pathway modulates brain arousal in freely behaving and isoflurane-anesthetized male mice

Fig. 5

a Schematic diagram of viral injection, optical fiber implantation, and EEG recording. b Verification of virus expression in VP Glu neurons. c An optical fiber was implanted in the LC. d−f Representative EEG traces (upper panel) and power spectra (lower) before, during, and after optical stimulation in ChR2 mice. (g−i) Power spectra before and during light stimulation in mice at different motor states. (j−l) % total power at EEG waves before and during light stimulation in mice at different motor states. Two-tailed paired t tests for (j−l). P-values are two-sided. Before vs during light illumination. n = 8 mice. Mean ± SEM in (g−l). The source data are in the Supplementary Dataset, Source Data.xlsx. Statistical results are in Supplementary Table 1.

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