Fig. 8: The VPGlu-LCNE-LH pathway regulates arousal and anesthesia through α2a receptor in the LH. | Nature Communications

Fig. 8: The VPGlu-LCNE-LH pathway regulates arousal and anesthesia through α2a receptor in the LH.

From: A ventral pallidum-locus coeruleus-lateral hypothalamus pathway modulates brain arousal in freely behaving and isoflurane-anesthetized male mice

Fig. 8

a Schematic diagram of virus injection, cannula implantation, and EEG recording in DBH-Cre mice. b, c Images showing virus expression in the LC and a cannula in the LH. d−f % time, number of episodes, and total duration in each motor state between 1 to 1.5 h after Vehicle, CNO and CNO + Yohembine (Yoh) injection. g−i Representative traces of EEG signals, % power of EEG waves, and EEG power spectrum between 0.5 and 1.5 h after injection of vehicle, CNO, or CNO+Yoh into the LH, in freely behaving mice. j−m Time to LORR in 1.4% isoflurane and RORR and motor score recovery after 20 min anesthesia with 1.4% isoflurane after delivery of Vehicle, CNO and CNO+Yoh into the LH. One-way repeated measures ANOVAs in (d−f, h, j, k, m). P-values are two-sided. n = 6 in each group. Mean ± SEM in (d−f, h−m). The source data are in the Supplementary Dataset, Source Data.xlsx. Statistical results are in Supplementary Table 1.

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