Fig. 2: PVN oxytocinergic neuronal activity was decreased during METH-CPP expression.

A Experimental timeline. B Schematic of virus injection. C Overlap of mCherry (red) and OXT (green). Scale bar: 50 μm. D Percentage of mCherry and OXT co-localizations (n = 6, two-sided Student’s t test). E Overlap of mCherry (red) and cFos (green) in the saline (left) and METH (right) group. Scale bar: 50 μm. F Percentage of mCherry and cFos co-localizations (n = 4 Saline/5 METH, two-sided Student’s t test). G OXT protein concentration in the PVN (n = 6 Saline/11 METH, two-sided Student’s t test). *p < 0.05, ***p < 0.001. Source data are provided as a Source Data file.