Fig. 6: Skewed differentiation of B cells and clonal expansion of plasmablasts in VEXAS. | Nature Communications

Fig. 6: Skewed differentiation of B cells and clonal expansion of plasmablasts in VEXAS.

From: In depth transcriptomic profiling defines a landscape of dysfunctional immune responses in patients with VEXAS syndrome

Fig. 6

a A UMAP plot of 11,335 B cells. b A neighborhood (Nhood) graph of B cells, which was generated similarly as in Fig. 2a. c Beeswarm and box plots of each B cell subtype for VEXAS (n = 9) and healthy donors (n = 5), which were generated similarly as in Fig. 2b. d Proportions of B cell subtypes relative to the total number of B cells in VEXAS (n = 11, red dots) and healthy donors (n = 8, blue dots) by flow cytometry. Data are presented as mean with SD. P values were calculated with the two-sided unpaired Mann–Whitney U test. e UMAP plots of B cells from VEXAS patients (right) and healthy donors (left) colored by clonal expansion sizes. f Distributions of B cell clone states in each subtype in VEXAS patients and healthy donors. g Isotype distributions of immunoglobulins expressed by each transcriptionally defined B cell subpopulation within all subjects. h A bar chart showing gene set enrichment scores of top 10 upregulated hallmark pathways in plasmablasts from VEXAS patients. A horizontal axis displays normalized enrichment score (NES) differences between VEXAS patients and healthy donors. A color scale indicates FDR values. BCR B-cell receptor. Source data are provided as a Source Data file.

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